“Certainty is a sure sign of a pathological mind…”
B W Powe
Colour is important. Clever choice of colour can provide definition, interest, depth and mood. Colour can make things pop and not just in art. Histological interpretation of tissue samples will be important to all of us one day. To surgeons it is important virtually every day. We need a correct diagnosis, assessment of the success of our work and a heads up about how a disease, particularly tumours, can be expected to behave. The classic tissue stain in pathology is H&E. Haematoxylin and Eosin is actually two dyes, developed independently in 1865 and 1875, respectively by Bohmer and Fischer. In 1876, Wissowzky had the bright idea to combine them so that dull tissue sections would be easier to interpret. Haematoxylin is a cationic aluminium salt and acts as a basic dye. It stains nucleic acids in the cell nucleus a blue/purple hue. Eosin is anionic and an acidic dye. It is negatively charged and stains protein amino groups in cell cytoplasm bright pink. #thebasisofwhatido #letsmakelemonade
Pen Paint and Scalpel 